An unanswered question in public health is whether anti-oxidation prevents or promotes cancer. Although anti-oxidation has historically been viewed as chemo-preventive, emerging evidence suggests that antioxidants can actually support neoplasia. We posit that this contention is rooted in the fact that reactive oxygen species (ROS) do not operate indiscriminately, but rather, function as selective, diverse secondary messengers with distinct functions. By functionally interrogating individual redox switches in PDA cells and the surrounding tumor microenvironment,  our laboratory aims to test the therapeutic principle that the specific redox dependencies of PDA cells can be exploited to target tumor cells while sparing normal tissues. 

 Pancreatic ductal organoids-- An ex-vivo 3D culture platform that we use to interrogate biological dependencies in PDA

Pancreatic ductal organoids-- An ex-vivo 3D culture platform that we use to interrogate biological dependencies in PDA

 Optical probes to assess the dynamics of specific redox couples in organoids and in vivo

Optical probes to assess the dynamics of specific redox couples in organoids and in vivo

 Organoid-stromal cell co-cultures to probe redox-dependent cancer-tumor microenvironment interactions

Organoid-stromal cell co-cultures to probe redox-dependent cancer-tumor microenvironment interactions

 Mass spectrometric approaches are used to probe oxidative post-translational modifications of the cysteine proteome and its implications in the growth and development of pancreatic cancer 

Mass spectrometric approaches are used to probe oxidative post-translational modifications of the cysteine proteome and its implications in the growth and development of pancreatic cancer 

 In vivo model systems (autochthonous and organoid transplant mouse models) are used to probe the therapeutic relevance of different intervention strategies.

In vivo model systems (autochthonous and organoid transplant mouse models) are used to probe the therapeutic relevance of different intervention strategies.

 © Copyright 2017-2018 by Christine Chio Lab. All Rights Reserved.

© Copyright 2017-2018 by Christine Chio Lab. All Rights Reserved.